Embryo vitrification

This kind of procedure, like vitrification of embryos, is one of the methods of cryopreservation (freezing). It is used when it is necessary to postpone the IVF protocol. With the introduction of this method, it was possible to significantly improve the survival rate of both sex cells and embryos after the thawing procedure.

When does it become necessary to freeze embryos?

It should be noted that cryopreservation can be carried out at any stage of development (pronucleus, crushing embryo, blastocyst). Because the procedure can be used at almost any time, when there is a probability of unsuccessful carrying out of landing in the uterus.

As for the immediate benefits of freezing, among these should be called:

1. Increased probability of pregnancy after IVF and prevention of death of normal viable embryos, which makes it possible to use them after in vitro fertilization.
2. It prevents the effects of hyperstimulation in the presence of a high probability of its development.
3. It is a solution to the problem in which the synchronization of the menstrual cycles of the donor and the recipient is impossible.

The cryopreservation of embryos by the vitrification method is mandatory when:

• decrease in the probability of implantation in the uterine cavity, in view of the presence of disorders in the reproductive organ (polyps, for example);
• stenosis of the cervical canal (no catheter can be inserted through the cervical canal).

How does freezing affect the embryo?

In the course of numerous experimental studies, it was found that carrying out this procedure has virtually no effect on the further development of the embryo. So, if necessary, the biomaterial is extracted from the capsule with liquid nitrogen, left at a temperature of 20-22 degrees, after which the cryoprotectant is removed and the embryo is placed in a special medium. After assessing the condition of the embryo, proceed to the planting procedure.